As chief physician to Roman gladiators and later to Emperor Marcus Aurelius, Galen of Pergamon conducted animal dissections and vivisections to study human anatomy. He identified nervous system functions, demonstrated that arteries carried blood rather than air, and developed experimental physiology methods. Galen combined Hippocratic principles with his observations to create a comprehensive medical system. His writings on anatomy, physiology, pathology, pharmacology, and medical philosophy dominated Western medicine for over 1,300 years. His observational and experimental methods influenced medical practice throughout the Roman Empire from approximately 160-200 CE.