Approximately 3.5 billion years ago, the earliest known self-replicating microorganisms emerged on Earth. These single-celled prokaryotes likely inhabited diverse aquatic environments near hydrothermal vents or shallow pools. Evidence comes from multiple sources: chemical isotope signatures in ancient rocks, microfossils, and stromatolite formations found in Western Australia and South Africa. The emergence of these microbes initiated biological processes that would alter Earth's atmosphere and surface chemistry over billions of years, establishing the conditions for all subsequent biological evolution.